人結(jié)腸癌細胞HCT116

人結(jié)腸癌細胞HCT116 是1979年BrattainM等分離建立的3株惡性細胞之一；產(chǎn)生CEA、角蛋白。在半固體瓊脂糖培養(yǎng)基中形成克隆。HCT116在無胸腺的裸鼠有致瘤性，形成上皮樣的腫瘤。

細胞名稱：人結(jié)腸癌細胞

細胞簡稱：HCT116細胞

細胞別稱：HCT-116; HCT.116; HCT_116; HCT116; CoCL2

產(chǎn)品貨號：TCH-C185

種屬來源：人

組織來源：結(jié)腸

特征：結(jié)腸癌

細胞形態(tài)：上皮細胞樣

生長特性：貼壁生長

培養(yǎng)體系： McCoy's 5A+10%FBS+1%P/S

配套培養(yǎng)基貨號：TCH-G185

傳代比例：1:3-1:8，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲存

質(zhì)量檢測：細菌、真菌、支原體檢測均為陰性

實驗耗材：細胞培養(yǎng)瓶，錐形搖瓶,細胞培養(yǎng)皿

實驗室儀器：CO2培養(yǎng)箱

人結(jié)腸癌細胞HCT116 使用說明書

參考文獻：

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