MIA-PaCa-2細(xì)胞
細(xì)胞名稱: 人胰腺癌細(xì)胞(STR鑒定正確)
細(xì)胞別名: MIA-PaCa-2; MIA-PACA-2; MIA-Pa-Ca-2; MIA Paca2;
MIA PaCa2; MiaPaCa-2; MIAPACA-2; MiaPaca.2; MiaPaCa2; Miapaca2; MIAPaCa2; MIAPACA2; Mia PACA 2; MIAPaCa-2; PaCa2
貨號(hào):TCH-C259
規(guī)格: 1X10^6
參考目錄價(jià)格:1600元 (干冰運(yùn)輸需另加200元干冰費(fèi))
種屬來源:人
組織來源:胰腺
特征:胰腺癌
細(xì)胞形態(tài):上皮細(xì)胞樣
生長(zhǎng)特性:貼壁生長(zhǎng)
培養(yǎng)體系: DMEM-H+10%FBS+2.5%馬血清+1% Sodium Pyruvate+1%P/S
MIA-PaCa-2細(xì)胞培養(yǎng)基貨號(hào):TCH-G259
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲(chǔ)存
質(zhì)量檢測(cè):細(xì)菌、真菌、支原體檢測(cè)均為陰性
該細(xì)胞表達(dá)人集落刺激因子CFS-I和血漿酶原激活劑。
MIA-PaCa-2細(xì)胞規(guī)格
參考文獻(xiàn):
Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590
Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740
Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455
Yunis AA, et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918
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