Jurkat細(xì)胞

細(xì)胞名稱： 人T淋巴細(xì)胞白血*細(xì)胞（STR鑒定正確）

細(xì)胞簡(jiǎn)稱：Jurkat細(xì)胞

細(xì)胞別稱：JurkatE6-1; Jurkat E6-1; Jurkat, Clone E6-1; Jurkat Clone E6-1; Jurkat (clone E6-1); JURKAT E-6.1; JURKAT E-61; Jurkat-E6; Jurkat E6; J.E6-1; E6-1

貨號(hào)：TCH-C225

規(guī)格： 1×10?cells/T25 培養(yǎng)瓶

價(jià)格：1500元   (干冰運(yùn)輸需另加200元干冰費(fèi))

種屬來(lái)源：人

組織來(lái)源：外周血

特征：急性T淋巴細(xì)胞白血*

細(xì)胞形態(tài)：淋巴母細(xì)胞樣

生長(zhǎng)特性：懸浮生長(zhǎng)

培養(yǎng)體系：RPMI-1640+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

Jurkat細(xì)胞 配套培養(yǎng)基貨號(hào)：TCH-G225

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：24-48 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

該細(xì)胞是Jurkat-FHCRC細(xì)胞株(Jurkat細(xì)胞株的衍生)的一個(gè)克隆。生長(zhǎng)方式為懸浮生長(zhǎng)，生長(zhǎng)速度較快，使用1640培養(yǎng)液培養(yǎng)。顯微鏡下觀察，正常細(xì)胞狀態(tài)為圓潤(rùn)透亮，似魚(yú)卵。

Jurkat細(xì)胞株來(lái)源于一個(gè)14歲男孩的外周血；經(jīng)弗波酯和外源凝集素或抗T3單克隆抗體中的一種誘導(dǎo)后可產(chǎn)生大量IL-2(IL-2的產(chǎn)生需兩種類型的誘導(dǎo)劑)；表達(dá)T細(xì)胞受體、CD3。

    蘇州阿爾法生物實(shí)驗(yàn)器材有限公司除了提供細(xì)胞系購(gòu)買(mǎi)，細(xì)胞株購(gòu)買(mǎi)，pc12細(xì)胞株，肺癌細(xì)胞株，Jurkat細(xì)胞，血管內(nèi)皮細(xì)胞株，肝癌細(xì)胞株，腫瘤細(xì)胞株，胃癌細(xì)胞株，hela細(xì)胞株，胰島細(xì)胞株，s180細(xì)胞株，hela細(xì)胞株，動(dòng)物細(xì)胞，干細(xì)胞外，還提供MEM培養(yǎng)基,DMEM,F12培養(yǎng)基,RPMI培養(yǎng)基等基礎(chǔ)培養(yǎng)基，干細(xì)胞培養(yǎng)基,培養(yǎng)基添加劑,間充質(zhì)干細(xì)胞培養(yǎng)基 ,培養(yǎng)基,低血清培養(yǎng)基,胎牛血清,雞血清等細(xì)胞培養(yǎng)試劑。

參考文獻(xiàn)：

"Weiss A, et al. The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL-2 production reflects events occurring at a pre-translational level. J. Immunol. 133: 123-128, 1984. PubMed: 6327821

Gillis S, Watson J. Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line. J. Exp. Med. 152: 1709-1719, 1980. PubMed: 6778951

Berninghausen O, Leippe M. Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect. Immun. 65: 3615-3621, 1997. PubMed: 9284127

Churchill MJ, et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNA expression in primate cells. J. Virol. 70: 5786-5790, 1996. PubMed: 8709194

Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663

Tiffany HL, et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842

Chan YJ, et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J. Virol. 70: 8590-8605, 1996. PubMed: 8970984

Kung SH, Medveczky PG. Identification of a herpesvirus saimiri cis-acting DNA fragment that permits stable replication of episomes in transformed T cells. J. Virol. 70: 1738-1744, 1996. PubMed: 8627695

Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082"