ActiveGαiGTP[26901][30µL]CatalogNumber:26901GeneSymbol:GnaiDescription:AntiActiveGαiMouseMonoclonalAntibodyBackground:HeterotrimericGproteinsarecriticalcellularsignaltransducers

詳細介紹

Active Gαi GTP [26901]
[30 µL]

Catalog Number: 26901

Gene Symbol: Gnai

Description: Anti Active Gα_i Mouse Monoclonal Antibody

Background: Heterotrimeric G proteins are critical cellular signal transducers. Gα_irepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα_i proteins are being explored.

Immunogen: Recombinant full length protein of active Gα_i1

Applications: IP, IHC

Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG1

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg ²⁺ａnd Ca ²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti active Gα_i antibody recognizes active Gα_i1, Gα_i2, and Gα_i3 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Immunoprecipitation/Western blot:

Gα_i activation assay.
A. CHO cells were

transfected with wild-type Gα_i1 (lanes 1 and 2) or constitutively

active Gα_i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS

(lane 3). Lysates were then incubated with an anti-active Gα_i monoclonal antibody

(Cat. No.26901) (top panel). The precipitated active Gα_i was immunoblotted with

an anti Gα_i monoclonal antibody (Cat. No. 26003). The bottom panel shows the

Western blot with anti Gα_i monoclonal antibody (Cat.No. 26003) of the cell lysates.

B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or

without (lane 1)carbachol. Cell lysates were then incubated with an anti active Gα_i monoclonal antibody

(Cat. No. 26901) (top panel).The precipitated active Ga _i was immunoblotted with an anti Gα_i rabbit polyclonal

antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti tubulin of the cell lysates.

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