Anti-IQCK抗體,IQCK蛋白抗體
英文介紹:Chromosome 16 encodes over 900 genes in approximay 90 million base pairs, makes up nearly 3% of human cellular DNA and is associated with a variety of genetic disorders. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth. The rare disorder Rubinstein-Taybi syndrome is also associated with chromosome 16, though through the CREBBP gene which encodes a critical CREB binding protein. Signs of Rubinstein-Taybi include mental retardation and predisposition to tumor growth and white blood cell neoplasias. Crohn's disease is a gastrointestinal inflammatory condition associated with chromosome 16 through the NOD2 gene. An association with systemic lupus erythematosis and a number of other autoimmune disorders with the pericentromeric region of chromosome 16 has led to the identification of SLC5A11 as a potential autoimmune modifier. alternatively splicing events.
此產(chǎn)品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-IQCK抗體,IQCK蛋白抗體轉膜(Transfer):
我們*在Western實驗中選用PVDF膜。硝酸纖維素膜(NC膜)或PVDF膜(二氟化樹脂膜膜孔徑:0.45um)也可以使用,但硝酸纖維素膜比較脆,在操作過程中特別是用鑷子夾取等過程中容易裂開。膜的使用請參考生產(chǎn)商的*使用步驟。
通常如果使用Bio-Rad的標準濕式轉膜裝置,可以設定轉膜電壓120V,轉膜時間為30-60分鐘。具體的轉膜時間要根據(jù)目的蛋白的大小而定,目的蛋白的分子量越大,需要的轉膜時間越長,目的蛋白的分子量越小,需要的轉膜時間越短。
在轉膜過程中,特別是高電流快速轉膜時,通常會有非常嚴重的發(fā)熱現(xiàn)象,把轉膜槽放置在冰浴中進行轉膜。轉膜的效果可以觀察所使用的預染蛋白質分子量標準,通常分子量zui大的1-2條帶較難全部轉到膜上。轉膜的效果也可以用麗春紅染色液對膜進行染色,以觀察實際的轉膜效果。也可以用考馬斯亮藍快速染色液對完成轉膜的SDS-PAGE膠進行染色,以觀察蛋白的殘留情況。
產(chǎn)品名稱:Anti-IQCK抗體,IQCK蛋白抗體
產(chǎn)品編號:BYK-9023R
英文名稱:Anti-IQCK
中文名稱:IQCK蛋白抗體
英文別名:FLJ20115; FLJ36575; IQ domain containing protein K; MGC35048; IQCK_HUMAN.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細胞術等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
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