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聯(lián)系我時,請告知來自 環(huán)保在線Anti-Phospho-HER3 (Tyr1222)磷酸化HER3受體抗體
Anti-Phospho-HER3 (Tyr1222)
磷酸化HER3受體抗體
專業(yè)提供Anti-Phospho-HER3 (Tyr1222)磷酸化HER3受體抗體,廣泛應(yīng)用于免疫組化,ELISA,Western Blot IHC-F(免疫組化-冰凍切片),IHC-P (免疫組化-石蠟切片)IP(免疫沉淀), Flow-Cyt等方面。
免疫組化實驗注意事項:
實驗所用主要為組織標(biāo)本和細胞標(biāo)本兩大類,前者包括石蠟切片(病理大片和組織芯片)和冰凍切片,后者包括組織印片、細胞爬片和細胞涂片。
其中石蠟切片是制作組織標(biāo)本zui常用、zui基本的方法,對于組織形態(tài)保存好,且能作連續(xù)切片,有利于各種染色對照觀察;還能*存檔,供回顧性研究;石蠟切片制作過程對組織內(nèi)抗原暴露有一定的影響,但可進行抗原修復(fù),是免疫組化中*的組織標(biāo)本制作方法。
產(chǎn)品編號 Rs-3219R
英文名稱 Anti-Phospho-HER3 (Tyr1222)
中文名稱 磷酸化HER3受體抗體
別 名 ERBB3; c erbB 3; c erbB3; ERBB3 protein; erbB3 S; Glial growth factor receptor; HER 3; HER3; LCCS2; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; proto-oncogene-like protein c ErbB 3; proto-oncogene-like protein c ErbB3; receptor tyrosine protein kinase ERB3; Receptor tyrosine protein kinase erbB 3; Receptor tyrosine protein kinase erbB3; Tyrosine kinase type cell surface receptor HER3.
背景介紹:ErbB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. Isoform 2 lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized. Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2); also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord.
產(chǎn)品類型 一抗 磷酸化抗體
抗體來源 Rabbit
克隆號 Polyclonal
交叉反應(yīng) hu, rat, mo
蛋白分子量 Predicted Molecular Weight: 148kDa
性狀 Lyophilized or Liquid
濃度 1mg/1ml
免疫原 KLH conjugated Synthesised phosphopeptide derived from human HER3 around the phosphorylation site of Tyr1222
亞型 IgG
純化方法 affinity purified by Protein A
儲存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
產(chǎn)品應(yīng)用 WB=1:100-500 Elisa=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1/20-1/100
(石蠟切片需做抗原修復(fù)) Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
研究領(lǐng)域 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 生長因子和激素 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 細胞類型標(biāo)志物
保存 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
公司供應(yīng)的 Anti-Phospho-HER3 (Tyr1222)磷酸化HER3受體抗體,已被國內(nèi)外廣大科研工作者使用,廣泛用于分子生物學(xué)、免疫學(xué)等試驗中。
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Anti-AATK(Apoptosis associated tyrosine kinase) 細胞凋亡關(guān)聯(lián)*激酶抗體
相關(guān)知識:
免疫組化的結(jié)果判斷有兩種方法:
一是對以檢測結(jié)果陽性細胞指數(shù)來定性(如核抗原的標(biāo)記),判斷方法是以一個視野中的陽性細胞數(shù)與總細胞的百分比,再取10個相同視野算取平均指數(shù)。
另一種方法以染色陽性強度和陽性檢出率相結(jié)合而定,一般陽性細胞數(shù)在0~25為陰性,25~50為十,50~75為十十,75以上為十十十。此種判定方法容易出現(xiàn)人為誤差現(xiàn)象。
有條件的實驗室能用圖像分析系統(tǒng)進行結(jié)果檢測定量分析更為準確。一切的判定方法都是力求使免疫組化染色結(jié)果判斷更標(biāo)準,但各單位采取的標(biāo)準不盡相同,所以判斷標(biāo)準化問題還有待*實踐中病理學(xué)術(shù)界商討判定標(biāo)準。
IHC中常見的抗原表達模式有以下幾種:
(1)細胞漿內(nèi)彌漫性分布,多數(shù)胞漿型抗體的反應(yīng)如此,如細胞角蛋白(cytokeratin,CK)和波形蛋白(vimentin)等;
(2)細胞核周的胞漿內(nèi)分布,其判別要點是細胞核的輪廓被勾畫得很清楚,如CD3多克隆抗體的染色;
(3)胞漿內(nèi)局限性點狀陽性,如CDl5抗體的染色;
(4)細胞膜線性陽性,大多數(shù)淋巴細胞標(biāo)記的染色如此,如CD20、CD45RO;
(5)細胞核陽性,如Ki-67及雌、孕激素受體蛋白ER、PR等。一種抗體可同時出現(xiàn)細胞漿和細胞膜的陽性表達,如EMA可呈膜性和胞漿內(nèi)彌漫性陽性反應(yīng);CD30抗體可同時呈膜性和胞漿內(nèi)點狀陽性反應(yīng)等。
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