Rabbit Anti-Rho A 抗體說(shuō)明書(shū)
FROM: Santa Cruz
Anti-Rho A 抗體說(shuō)明書(shū)
Catalog Number : HZ98123
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins （H-, K- and N-Ras p21） are members of a much larger superfamily of related proteins. Six members of this family （Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B）, exhibit approximay 50% amino acid homology to Ras. The five mammalian Rho proteins （Rho A, B, C, 7 and 8） are approximay 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence （C, cysteine; A, aliphatic amino acid; X, any amino acid） which in the case of Ras has been shown to be essential for correct localization and function.
Anti-Rho A 抗體說(shuō)明書(shū)
pecificity :
– Anti-Rho A is a rabbit monoclonal antibody unconjugated
– specific for Rho A of vinegar fly ,human, mouse, rat
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 24 kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
