Description:

Goat anti-human Fibrinogen, HRP conjugated

Catalog Number:

bse-0363G

Quantity size:

0.1ml （2mg/ml; Buffer = 0.01M PBS, pH 7.4 with 10 mg/mL BSA and 0.1% Gentamicin, 50% glycerol.）

Purification Method:

Purified by Protein-A affinity chromatography.

Host:

Goat

Background Information:

Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A （1 to 16 amino acids） and B （1 to 17 amino acids） are released by thrombin from the N terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases.

Key Applications:

· Western blotting: 1:1000-5000

· Immunohistochemistry: 1:200-500

· Immunocytochemistry: 1:200-500

· ELISA: 1:1000-5000

· Optimal working dilutions must be determined by the end user.

Species Reactivity:

human Fibrinogen

Antibody Category:

Secondary & Control Antibodies

Antibody Sub-Category:

Whole Immunoglobulin Secondary Antibodies （H+L）

Storage:

Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4  0.01 M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ºC.

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.