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當(dāng)前位置:齊一生物科技(上海)有限公司>>細(xì)胞生物>>傳代細(xì)胞>> 人肺癌細(xì)胞A549
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更新時(shí)間:2021-12-23 09:17:47瀏覽次數(shù):303次
聯(lián)系我時(shí),請告知來自 環(huán)保在線人肺癌細(xì)胞A549
細(xì)胞名稱 人肺癌細(xì)胞A549 A549 [A-549]
形態(tài)特性 上皮樣;多角形
生長特性 貼壁生長
特征特性 該細(xì)胞系是1972年由Giard DJ通過肺癌組織移植培養(yǎng)建系的,源自一位58歲的白人男性。A549能通過胞苷二磷脂酰膽堿途徑合成富含不飽和脂肪酸的卵磷脂;角蛋白陽性。
培養(yǎng)條件 McCoy's 5A Media (Modified with Tricine) 10%FBS
傳代方法 1:3~1:8傳代;每周2~3次。
傳代情況 C5
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
支原體檢測 培養(yǎng)法(-)
STR Amelogenin:X,Y;CSF1PO:10,12;D13S317:11;D16S539:11,12;D18S51:14,17;D19S433:13;D21S11:29;D2S1338:24;D3S1358:16;D5S818:11;D7S820:8,11;D8S1179:13,14;FGA:23;TH01:8,9.3;TPOX:8,11;vWA:14;
同工酶
染色體 60~66,XY
人肺癌細(xì)胞;A549 [A-549]
PUMC——人肺癌細(xì)胞;A549 [A-549]
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