| Test principle: | ||||||||||||||||||||||||||||
| This assay employs a two-site sandwich ELISA to quantitate CAT in samples. An antibody specific for CAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAT bound in the initial step. The color development is stopped and the intensity of the color is measured. | ||||||||||||||||||||||||||||
| Product Overview: | ||||||||||||||||||||||||||||
| Components: | ||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||
| Specificity: | ||||||||||||||||||||||||||||
| This assay has high sensitivity and excellent specificity for detection of Rat CAT. No significant cross-reactivity or interference between Rat CAT and analogues was observed. | ||||||||||||||||||||||||||||
| Recovery: | ||||||||||||||||||||||||||||
Matrices listed below were spiked with certain level of recombinant Rat CAT and the recovery rates were calculated by comparing the measured value to the expected amount of Rat CAT in samples.
| ||||||||||||||||||||||||||||
| Precision: | ||||||||||||||||||||||||||||
| Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12% | ||||||||||||||||||||||||||||
| Linearity: | ||||||||||||||||||||||||||||
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat CAT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| ||||||||||||||||||||||||||||
| Stability: | ||||||||||||||||||||||||||||
| The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). | ||||||||||||||||||||||||||||
| Sample collection and storage: | ||||||||||||||||||||||||||||
| Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 2 - 8°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2 - 8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Other biological fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles. | ||||||||||||||||||||||||||||
| Kits storage instructions: | ||||||||||||||||||||||||||||
| Store at 2-8°C. Please refer to Instruction Manual. |
北京瑞達(dá)恒輝科技發(fā)展有限公司立足于生物技術(shù)前沿，是專業(yè)從事生命科學(xué)相關(guān)產(chǎn)品研發(fā)和銷售的綜合性生物化學(xué)公司。公司現(xiàn)擁有瑞達(dá)恒輝TM和HenghuibioTM兩個自有注冊品牌，并與國內(nèi)外多家生物技術(shù)品牌進(jìn)行廣泛合作。
長期以來，公司致力于生命科學(xué)技術(shù)產(chǎn)品的研究與開發(fā)，對生物下游目標(biāo)產(chǎn)物的分離純化做了大量的探索和實踐，客戶遍布清華、北大、中科大等科研院所、以及各類科技工業(yè)企業(yè)。并與國內(nèi)各地代理商密切合作，贏得了各界朋友的肯定及好評。
平臺主打產(chǎn)品為生物下游分離純化常用的層析色譜填料、層析柱、透析袋等，另外我公司還銷售標(biāo)準(zhǔn)品以及各類生化試劑和耗材。
在公司員工不斷的努力和廣大客戶的關(guān)懷下，我們的產(chǎn)品還會不斷的的完善，質(zhì)量還會不斷的優(yōu)化，我們將著力提升員工的素質(zhì)，打造更加專業(yè)更加高效的團(tuán)隊，堅持“以質(zhì)量求生存，以信譽(yù)求發(fā)展”的原則，提升客戶的滿意度，爭取為廣大客戶提供更為便捷高效的服務(wù)！
層析柱，透析袋，填料，生化試劑
Rat Catalase (CAT) ELISA Kit 產(chǎn)品信息
