上海研盟生物科技有限公司PEG3抗體-PEG3蛋白抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取。客服
簡單介紹:
中文名稱:PEG3蛋白抗體
英文名稱:Anti-PEG3
產(chǎn)品編號(hào):BYK-1870R
產(chǎn)品別名:Paternally-expressed gene 3 protein; Zinc finger and SCAN domain-containing protein 24; Kruppel-type zinc finger protein; paternally expressed 3; Paternally expressed gene 3; PEG3 protein; Pw1; Zn-finger protein Pw1; PEG3_HUMAN; Paternally-expressed gene 3 protein.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實(shí)驗(yàn)
產(chǎn)品價(jià)格:請(qǐng)報(bào)價(jià)
說明書:請(qǐng)?zhí)砑涌头苯铀魅?/p>
抗體來源:該指標(biāo)有兩種產(chǎn)品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應(yīng):請(qǐng)索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲(chǔ) 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PEG3抗體-PEG3蛋白抗體產(chǎn)品介紹:In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.
多肽的溶解與保存
一、凍干多肽的溶解
1、多肽溶解的主要問題是二級(jí)結(jié)構(gòu)的形成。雖然疏水肽鏈二級(jí)結(jié)構(gòu)的形成更明顯,但除zui短的肽鏈外,此現(xiàn)象產(chǎn)生在幾乎所有肽鏈,與極性無關(guān)。所以,溶解多肽的*個(gè)原則是使用無菌蒸餾水或去離子水,當(dāng)然無氧水。多肽溶液可能遇到細(xì)菌降解,為防止此情況的發(fā)生,應(yīng)溶解在無菌的蒸餾水中或用0.45或0.2孔徑的濾膜過濾除菌。含有Cys、Met、Trp的多肽很容易氧化,應(yīng)溶于無氧的水中,無氧水可通過注入惰性氣體(氮、氦、氬)減壓除氣得到;
2、若多肽不溶于純水,超聲處理有助于打碎顆粒并增加溶解度。注意:超聲處理會(huì)引起溶液發(fā)熱或多肽降解;
3、若多肽含有多個(gè)堿性氨基酸,使用(1-10%)的乙酸水溶液:對(duì)于疏水性強(qiáng)的多肽,應(yīng)使用50%的乙酸;
4、若多肽中含有大量酸性氨基酸,可用氨溶液(1-10%)或乙酸乙基*或重碳酸鹽等揮發(fā)性的堿性緩沖液溶解。pH值在層析前必須調(diào)整;
5、異丙醇和乙腈能溶解中等大小的多肽。若肽要上柱,有機(jī)溶劑的量必須很少否則將嚴(yán)重影響停留時(shí)間;
6、若多肽因含有Val、Leu、Met、Phe、Tyr、Ala等芳香鏈而高度疏水、或?yàn)橹行噪臅r(shí),DMF或DMSO等膜變性劑的使用,有利于多肽的溶解:
a.高濃度模變性劑通過破壞多肽的二級(jí)結(jié)構(gòu)而助溶;
b.膜變性劑適于多肽分析液的制備,但可能會(huì)對(duì)其生物活性的研究工作造成干擾;
c.DMF是變性劑(zui高濃度可達(dá)30%),滴加至多肽溶解;
d.反相層析法時(shí),DMF將與洗脫液前鋒一起流出,根據(jù)入量的多少,峰值可能很高。大多數(shù)肽能在大量DMF流出后幾分鐘內(nèi)流出,若肽鏈很小,洗脫太早,多肽的量將很低。
相關(guān)產(chǎn)品:
鈣粘蛋白相關(guān)的神經(jīng)受體1(CNR-1)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
*加壓素(AVP)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
毛細(xì)血管擴(kuò)張性共濟(jì)失調(diào)突變基因(ATM)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
酸性神經(jīng)鞘磷脂酶(ASM)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
花生四烯酸5脂加氧酶(ALOX-5)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
5脂加氧酶(5-LO/LOX)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
芳香烴受體(AhR)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
晚期糖基化終末產(chǎn)物受體(RAGE/AGER)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
特異性巨噬細(xì)胞武裝因子(SMAF)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
促有絲分裂因子(MF/MPF)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
腺苷酸環(huán)化酶1(AC-1)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
可溶性腺苷酸環(huán)化酶(sAC)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
抗鼠抗體(HAMA)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
鈣離子通道抗體(VGCC)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
溴脫氧核苷*(BrdU)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
胃粘液素(GM)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
纖維母細(xì)胞表面抗原(FSP)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
轉(zhuǎn)移因子(TF)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
*脫羧酶(ODC)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
黑色素瘤相關(guān)抗原(MAGE)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
前膠原C端肽酶(PCP)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
多形核白細(xì)胞彈性蛋白酶(PMN Elastase)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
補(bǔ)體片斷3b(C3b)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)
補(bǔ)體片斷4b(C4b)ELISA試劑盒,96T/48T科研實(shí)驗(yàn)