上海研盟生物科技有限公司phospho-FSCN1抗體-磷酸化纖維束蛋白同源物1抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細胞術(shù)等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/p>
簡單介紹:
中文名稱:磷酸化纖維束蛋白同源物1抗體
英文名稱:Anti-phospho-FSCN1
產(chǎn)品編號:BYK-0772R
產(chǎn)品別名:Fascin (phospho S39); Fascin (phospho S39); 55 kDa actin bundling protein; Actin bundling protein; FAN 1; FAN1; Fascin 1; Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus); Fascin homolog 1; Fascin1; FLJ38511; FSCN 1; FSCN1; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); Singed drosophila homolog like; Singed like (fascin homolog sea urchin) (Drosophila); Singed like protein; SNL; Strongylocentrotus purpuratus.
產(chǎn)品規(guī)格:0.1ml、0.2ml、1ml
產(chǎn)品用途:科研實驗
產(chǎn)品價格:請報價
說明書:請?zhí)砑涌头苯铀魅?/p>
抗體來源:該指標(biāo)有兩種產(chǎn)品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應(yīng):請索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-FSCN1抗體-磷酸化纖維束蛋白同源物1抗體產(chǎn)品介紹:Human fascin is a highly conserved actin-bundling protein. Fascin, encoded by the human homolog for sn (hsn) gene, has been localized to microspikes and stress fibers of cultured cells where it is thought to be involved in the formation of microfilament bundles. It is expressed predominantly in dendritic cells. Lymphoid cells, myeloid cells and plasma cells are negative. However, Reed Sternberg cells in Hodgkin’s lymphoma are positive for fascin staining. Epstein-Barr virus may induce expression of fascin in B cells.
設(shè)置對照
其目的在于證明和肯定陽性結(jié)果的特異性,排除非特異性疑問。主要是針對*抗體對照,常用的對照方法包括:1. 陽性對照;2. 陰性對照;3. 阻斷實驗; 4.替代對照;5. 空白對照;6. 自身對照;7. 吸收實驗.
1. 陽性對照用已知抗原陽性的切片作對照與待檢標(biāo)本同時進行免疫組織化學(xué)染色,對照切片應(yīng)呈陽性結(jié)果,成為陽性對照。證明全過程均符合要求,尤其當(dāng)待檢標(biāo)本呈現(xiàn)陰性結(jié)果時,陽性對照尤為重要。
2.陰性對照用確證不含已知抗原的標(biāo)本作對照,應(yīng)呈陰性結(jié)果,成為陰性對照,是陰性對照的一種。其實空白、替代、吸收和抑制都屬于陰性對照。當(dāng)待檢標(biāo)本呈陽性結(jié)果時,陰性對照就更加重要,用以排除假陽性。
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