Anti-Gcn1l1抗體,廣泛控制氨基酸合成1樣蛋白1抗體孵育(Primary antibody incubation):
一抗孵育(Primary antibody incubation)
參考一抗的說明書,按照適當(dāng)比例用Western一抗稀釋液稀釋一抗。
用微型臺(tái)式真空泵吸盡封閉液,立即加入稀釋好的一抗,室溫或4℃在側(cè)擺搖床上緩慢搖動(dòng)孵育一小時(shí)。如果一抗孵育一小時(shí)效果不佳,可以在4℃緩慢搖動(dòng)孵育過夜?;厥找豢埂<尤隬estern洗滌液,在側(cè)擺搖床上緩慢搖動(dòng)洗滌5-10分鐘。吸盡洗滌液后,再加入洗滌液,洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長洗滌時(shí)間并增加洗滌次數(shù)。
二抗孵育(Secondary antibody inucubation)
參考二抗的說明書,按照適當(dāng)比例用Western二抗稀釋液稀釋辣根過氧化物酶(HRP)標(biāo)記的二抗。
用微型臺(tái)式真空泵吸盡洗滌液,立即加入稀釋好的二抗,室溫或4℃在側(cè)擺搖床上緩慢搖動(dòng)孵育一小時(shí)。回收二抗,加入Western洗滌液,在側(cè)擺搖床上緩慢搖動(dòng)洗滌5-10分鐘。吸盡洗滌液后,再加入洗滌液,洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長洗滌時(shí)間并增加洗滌次數(shù)。
產(chǎn)品名稱:Anti-Gcn1l1抗體,廣泛控制氨基酸合成1樣蛋白1抗體
產(chǎn)品編號(hào):BYK-13315R
英文名稱:Anti-Gcn1l1
中文名稱:廣泛控制氨基酸合成1樣蛋白1抗體
英文別名:GCN1; GCN1 (general control of amino acid synthesis yeast homolog) like; GCN1 (general control of amino acid synthesis 1 yeast) like 1; GCN1 general control of amino acid synthesis 1 like 1; GCN1 general control of amino acid synthesis 1 like 1 (yeast); GCN1 like protein 1; GCN1L; HsGCN1; GCN1L_HUMAN.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-Gcn1l1抗體,廣泛控制氨基酸合成1樣蛋白1抗體
英文介紹:GCN1L1 is a 2,671 amino acid protein that is ubiquitously expressed and belongs to the GCN1 family. Functioning as a translation activator, GCN1L1 interacts with IMPACT to regulproteinate GCN2 kinase activity. GCN1L1 contains 24 HEAT repeats and is encoded by a gene that maps to human chromosome 12q24.23. Chromosome 12 encodes over 1,100 genes and comprises approximay 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and trisomy 12p, which causes facial developmental defects and seizure disorders.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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